OMICSDESKbioinformatics, delivered

Sample deliverables

Every one of these was run end to end on public data, and the script that produced it is downloadable. Nothing here is illustrative: if a number appears, it came out of the pipeline — including the ones that are inconvenient for us.

Single-cell RNA-seq — the standard analysis

2,638 cells, 6 clusters, 0% unassigned — and the annotation cross-checked blind against a reference classifier (6/6 agreement).

Spatial transcriptomics — 10x Visium

4,025 spots, 10 spatial domains; the top spatially variable genes recover known lymph-node architecture without being told what to look for.

Bulk RNA-seq — differential expression

DESeq2 on public GEO data with the design read from the source metadata; milk-protein genes top the result, which is the biological check that the pipeline behaved.

Pseudobulk vs per-cell — the statistics decide the answer

On 8-donor data the two approaches disagree on 68.3% of differential-expression calls; all 10 canonical interferon genes are recovered by the design-aware analysis.

Batch integration — with the over-correction check

Donor clustering reduced 20.2% while the treatment signal moved -0.1%. Most integration reports show only the first number.

Answering reviewer comments — with computation

QC thresholds re-run at three settings (ARI 0.894/0.872); the permissive setting recovers a platelet population the strict one deletes. Plus real doublet detection.

Differential abundance — without inventing findings

"Cell type X increased after treatment" is the claim most often made wrongly. This one is tested at subject level.

The deliverable itself

A self-contained report as it actually arrives: figures embedded, QC table, annotation cross-check, a manuscript-ready methods paragraph, and a stated limitations section.

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