Spatial data is not "single-cell with coordinates". What is valid depends on whether your unit is a cell or a mixture — and we say which, in writing, before starting.
Run on public data, with the script downloadable.
Run the free QC report on your own data — no sign-up, and the file is deleted when the report is built. Or build a plan to see whether your design supports the comparison you have in mind; it will tell you when it does not.